Molecular Assay Catalogue
In our Molecular Assay Catalogue, we have compiled a list of local translational research capabilities and core facilities at partner cancer research institutes in Singapore. These assays are accessible to the Singapore cancer research community and STCC collaborators.
Research Use Only (RUO) assays
Genomics
Symbols: 🅢 spatial resolution ⁂ single-cell resolution
| Assay | Platform | Description | Service partner | |
|---|---|---|---|---|
| Whole Exome Sequencing (WES) | NGS, Illumina NextSeq 500/550 | Targeted sequencing of protein-coding regions. | A*STAR CGD POLARIS | |
| NGS, MGI G400 | Targeted sequencing of protein-coding regions. | NCCS CDH | ||
| Whole Genome Sequencing (WGS) | NGS, Illumina NextSeq 500/550 | Comprehensive whole-genome sequencing. | A*STAR CGD POLARIS | |
| NGS, MGI G400 | Comprehensive whole-genome sequencing. | NCCS CDH | ||
| Bulk RNA Sequencing (RNA-seq) | NGS, Illumina NextSeq 500/550 | Gene expression analysis from bulk samples. | A*STAR CGD POLARIS | |
| NGS, MGI G400 | Gene expression analysis from bulk samples. | NCCS CDH | ||
| Targeted RNA Sequencing | Nanostring nCounter | Targeted gene expression analysis with Nanostring panels. | NCCS CDH | |
| Agilent Magnis | Whole-exome RNA sequencing for FFPE samples. | NCCS CDH | ||
| Targeted Genome Sequencing | Agilent Magnis | Targeted DNA/RNA panel sequencing (Asian Pancancer Panel). | NCCS CDH | |
| Single-cell RNA Sequencing (scRNA-seq) | 10X Chromium | ⁂ | Single-cell resolution whole-transcriptomic profiling. | NCCS CDH |
| Spatial Transcriptomics | Nanostring GeoMx, nCounter | 🅢 | Area-based analysis of the entire human transcriptome. | NUS CSI MMA Core |
| 10X Visum + CytAssist | 🅢 | Whole-transcriptomic profiling at 55 µm resolution. | NCCS CDH | |
| BGI Stereoseq | Single-cell spatial whole-transcriptomic profiling. | NCCS CDH | ||
| DNA Methylation Profiling | Illumina (Twist EM-Seq) | 🅢 ⁂ |
Profiling methylated regions in the human genome. | NCCS CDH |
| Metagenomic Profiling | Illumina (Twist Panviral) | Profiling of 1,000+ viral human pathogens DNA samples. | NCCS CDH | |
| Circulating Tumor Cells (CTCs) | DEPArray | ⁂ | Isolation of circulating tumor cells (CTCs) for profiling. | NCCS CDH |
Service partners: A*STAR CGD POLARIS - Agency for Science, Technology and Research - Centre for Genome Diagnostics, Personalized OMIC Lattice for Advanced Research and Improving Stratification NCCS CDH - National Cancer Centre Singapore, Cancer Discovery Hub NUS CSI MMA Core - National University of Singapore, Cancer Science Institute of Singapore, Microscopy and Multiplex Assays Core Methods: EM-Seq - Enzymatic Methyl-sequencing NGS - Next Generation Sequencing FFPE - Formalin-Fixed Paraffin-Embedded |
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Proteomics
Symbols: 🅢 spatial resolution ⁂ single-cell resolution
| Assay | Platform | Description | Service partner | |
|---|---|---|---|---|
| Chromogenic Immunohistochemistry (IHC) | Leica Bond-MAX | 🅢 ⁂ |
Visualization of proteins in tissues using antibodies. | NUS CSI MMA Core |
| Multiplex Chromogenic IHC | Leica Bond-MAX | 🅢 ⁂ |
Up to 4 targets at sub-cellular resolution. | NUS CSI MMA Core |
| Multiplex Fluorescent IHC | Akoya PhenoImager HT | 🅢 ⁂ |
Up to 8 targets at sub-cellular resolution. | NUS CSI MMA Core |
| ZEISS Axioscan 7 | 🅢 ⁂ |
Up to 6 targets at sub-cellular resolution. | A*STAR IMCB | |
| Hyperplex Fluorescent IHC | Lunaphore COMET | 🅢 ⁂ |
Up to 40 targets at sub-cellular resolution. | A*STAR IMCB |
| Akoya Phenocycler | 🅢 ⁂ |
Up to 100+ targets at sub-cellular resolution. | NUS CSI MMA Core | |
| Miltenyi MACSima | 🅢 ⁂ |
Up to 50+ targets at sub-cellular resolution. | Miltenyi Biotec | |
| Spatial Proteomics | Nanostring GeoMx | 🅢 | Area-based analysis of up to 570 protein targets. | NUS CSI MMA Core |
| Multiplexed Flow Cytometry | CyTEK Aurora | ⁂ | Immune profiling with a 39-plex panel. | A*STAR IMCB |
| Serum Cytokine Profiling | Luminex xMAP | Multiplexed analysis of cytokines, chemokines, and growth factors. | A*STAR IMCB | |
| Secreted Protein Analysis | Isoplexis IsoLight | ⁂ | Ultra-high multiplex secreted protein analysis. | A*STAR IMCB |
Service partners: NUS CSI MMA Core - National University of Singapore, Cancer Science Institute of Singapore, Microscopy and Multiplex Assays Core A*STAR IMCB - Agency for Science, Technology and Research - Institute of Molecular and Cell Biology | ||||
Clinical (CAP-accredited) assays
Genomics
| Assay | Platform | Description | Service partner | |
|---|---|---|---|---|
| POLARIS® Clinical Exome Test (Technical) | NGS, Illumina NextSeq 500/550 | Targeted sequencing of protein-coding regions. | A*STAR CGD POLARIS | |
| POLARIS Somatic Cancer Test | NGS, Illumina NextSeq 500/550 | Detection of somatic mutations in 70 genes (lung, breast, prostate, colon). | A*STAR CGD POLARIS | |
| Alternative Promoter Score (APS) Test | NGS, Illumina NextSeq 500/550 | Test predicting response to immuno-therapy in gastric cancer. | A*STAR CGD POLARIS | |
| POLARIS Droplet Digital PCR (DdPCR) EGFR Mutation Test | ddPCR | EGFR mutation test for lung cancer. | A*STAR CGD POLARIS | |
| POLARIS RSPO Gene Fusion TEST | RT-qPCR | Detection of gene fusions involving RSPO2/3 for colorectal cancer. | A*STAR CGD POLARIS | |
| POLARIS Sanger Sequencing TEST | Sanger sequencing | Confirmation of mutation status via Sanger sequencing technology. | A*STAR CGD POLARIS | |
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Service partners: A*STAR CGD POLARIS - Agency for Science, Technology and Research - Centre for Genome Diagnostics, Personalized OMIC Lattice for Advanced Research and Improving Stratification Methods: NGS - Next-Generation Sequencing ddPCR - Droplet Digital Polymerase Chain Reaction RT-qPCR - Reverse Transcription Quantitative Polymerase Chain Reaction |
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